Control of porphyrin biosynthesis in Rhodopseudomonas spheroides and Propionibacterium shermanii. A direct 13C nuclear-magnetic-resonance spectroscopy study.
نویسندگان
چکیده
The facultative anaerobes Rhodopseudomonas spheroides and Propionibacterium shermanii were grown under anaerobic and aerobic conditions. The effect of light was studied with the photosynthetic R. spheroides, and the adaptation of both species to dark anaerobic life was monitored by direct observation of 5-amino[5-13C]laevulinic acid metabolism by using 13C nuclear-magnetic-resonance spectroscopy.
منابع مشابه
[ C ’ NjRiboflavin as Precursor in the Biosynthesis of the 5 , 6 - Dimethylbenzimidazole Moiety of Vitamin B 12
The 5,6-dimethylbenzimidazole formed from riboflavin in cobalt-containing cells of Propionibacterium shermanii can only be isolated as a component of vitamin Blz. No free 5,6-dimethylbenzimidazole is detectable in these bacteria. In order to determine, if nevertheless the vitamin Bn biosynthesis proceeds via free 5,6-dimethylbenzimidazole, cells of P. shermanii were incubated with [5-‘“Nlribofl...
متن کاملRiboflavin as precursor in the biosynthesis of the 5,6-Dimethylbenzimidazole-moiety of vitamin B(12).
The 5,6-dimethylbenzimidazole formed from riboflavin in cobalt-containing cells of Propionibacterium shermanii can only be isolated as a component of vitamin Blz. No free 5,6-dimethylbenzimidazole is detectable in these bacteria. In order to determine, if nevertheless the vitamin Bn biosynthesis proceeds via free 5,6-dimethylbenzimidazole, cells of P. shermanii were incubated with [5-‘“Nlribofl...
متن کاملThe Inhibition of Bacteriochlorophyll Biosynthesis in Rhodopseudomonas Spheroides by 8-hydroxyquinoline.
Granick (1948, 1950, 1961) has isolated different mutants of Chlorella which are unable to synthesize chorophyll and which accumulate magnesium protoporphyrin, magnesium vinyl phaeoporphyrin a5 and a monoester of magnesium protoporphyrin, presumed to be the monomethyl ester. Granick (1961) has proposed that these compounds are intermediates in chlorophyll biosynthesis, and it has been suggested...
متن کاملAssessing the quantitative reliability of solid-state 13C NMR spectra of kerogens across a gradient of thermal maturity.
Five type II kerogens, shown by elemental analysis and Rock-Eval pyrolysis to represent a gradient of thermal maturity, were further characterized using a range of solid-state 13C NMR spectroscopic techniques. 13C cross polarization (CP) NMR spectra of the kerogens confirmed the well-established pattern of increasing aromaticity with increasing thermal maturity. Spin counting showed that CP obs...
متن کاملAnalysis of Cerebrospinal Fluid in Diagnosis of Bacterial Meningitis; Using Nuclear Magnetic Resonance Spectroscopy: A Systematic Review
Background: Analysis of biofluids provides a unique window into the biochemical status of a living organism since the composition of a given biofluid will be modulated according to the level of function of the cells that are intimately concerned with its manufacture and secretion. One of the most successful approaches to biofluid analysis has been the application of NMR spectroscopy. Objective...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Biochemical journal
دوره 194 2 شماره
صفحات -
تاریخ انتشار 1981